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  • Record 1 of 17
    Title: The prevalence of ataxia telangiectasia mutated (ATM) variants in patients with breast cancer patients: a systematic review and meta-analysis
    Author(s): Moslemi, M (Moslemi, Masoumeh); Vafaei, M (Vafaei, Maryam); Khani, P (Khani, Pouria); Soheili, M (Soheili, Marzieh); Nedaeinia, R (Nedaeinia, Reza); Manian, M (Manian, Mostafa); Moradi, Y (Moradi, Yousef); Sohrabi, E (Sohrabi, Ehsan)
    Source: CANCER CELL INTERNATIONAL  Volume: 21  Issue: 1  Article Number: 474  DOI: 10.1186/s12935-021-02172-8  Published: SEP 8 2021  
    Abstract: Breast cancer is the most common cancer in women, and its high mortality has become one of the biggest health problems globally. Several studies have reported an association between breast cancer and ATM gene variants. This study aimed to demonstrate and analyze the relationship between ATM gene polymorphisms and breast cancer prevalence rate. A systematic literature review was undertaken using the following databases: Medline (PubMed), Web of sciences, Scopus, EMBASE, Cochrane, Ovid, and CINHAL to retrieve all cross-sectional studies between January 1990 and January 2020, which had reported the frequency of ATM variants in patients with breast cancer. A random-effects model was applied to calculate the pooled prevalence with a 95% confidence interval. The pooled prevalence of ATM variants in patients with breast cancer was 7% (95% CI: 5-8%). Also, the pooled estimate based on type of variants was 6% (95% CI: 4-8%; I square: 94%; P: 0.00) for total variants, 0% (95% CI: 0-1%; I square: 0%; P: 0.59) for deletion variants, 12% (95% CI: 7-18%; I square: 99%; P: 0.00) for substitution variants, and 2% (95% CI: 4-9%; I square: 67%; P: 0.08) for insertion variants. This meta-analysis showed that there is a significant relationship between ATM variants in breast cancer patients. Further studies are required to determine which of the variants of the ATM gene are associated with BRCA mutations.
    Accession Number: WOS:000693657000001
    PubMed ID: 34493284
    Author Identifiers:
    Moradi, Yousef    0000-0002-2936-5930 
    eISSN: 1475-2867
    Record 2 of 17
    Title: A Computational Framework to Infer Prostate Cancer-Associated Long Noncoding RNAs and Analyses for Identifying a Competing Endogenous RNA Network
    Author(s): Sajjadi, RS (Sajjadi, Roshanak S.); Modarressi, MH (Modarressi, Mohammad Hossein); Akbarian, F (Akbarian, Fahimeh); Tabatabaiefar, MA (Tabatabaiefar, Mohammad Amin)
    Source: GENETIC TESTING AND MOLECULAR BIOMARKERS  Volume: 25  Issue: 9  Pages: 582-589  DOI: 10.1089/gtmb.2021.0053  Published: SEP 1 2021  
    Abstract: Background: Prostate cancer (PC) is the second leading cause of cancer death after lung cancer in men. Current biomarkers are ineffective for the treatment and management of the disease. Long noncoding RNAs (lncRNAs) are a heterogeneous group of transcripts that are involved in complex gene expression regulatory networks. Although lncRNAs have been suggested to be promising as future biomarkers, the connection between the majority of lncRNAs and human disease remains to be elucidated. One approach to elucidate the roles of lncRNAs in disease is through the development of computational models. For example, a novel computational model termed HyperGeometric distribution for LncRNA-Disease Association (HGLDA) has been developed. Such models need to be developed on a tumor-specific basis to better suit the particular problem. Methods: In this study, we constructed a potential pipeline through two models, HGLDA and pathway-based using data from several databases. To validate the obtained data, the expression levels of selected lncRNAs were investigated quantitatively in the DU-145, LNCaP, and PC3 PC cell lines using quantitative real-time PCR. Results: We obtained a number of lncRNAs from both models, many of which were filtered through several databases that ultimately resulted in identification of six high-value lncRNA targets. Their expression was correlated with one important component of the PI3K pathway, known to be related to PC. Conclusion: Through the assembly of a lncRNA-miRNAs-mRNA competing endogenous RNA network, we successfully predicted lncRNAs interfering with miRNAs and coding genes related to PC.
    Accession Number: WOS:000697799900003
    PubMed ID: 34550779
    ISSN: 1945-0265
    eISSN: 1945-0257
    Record 3 of 17
    Title: JPX and LINC00641 ncRNAs expression in prostate tissue: a case-control study
    Author(s): Sajjadi, RS (Sajjadi, Roshanak S.); Modarressi, MH (Modarressi, Mohammad Hossein); Tabatabaiefar, MA (Tabatabaiefar, Mohammad Amin)
    Source: RESEARCH IN PHARMACEUTICAL SCIENCES  Volume: 16  Issue: 5  Pages: 493-504  DOI: 10.4103/1735-5362.323916  Published: SEP-OCT 2021  
    Abstract: Background and purpose: Prostate cancer (PC) is the second most prevalent cancer in men. Prostate-specific antigen (PSA) is the main biomarker for screening PC. An increase in PSA could lead to false-positive results. Thus, more appropriate markers should be investigated. In the present study, JPX and LINC00641 expression levels were measured in tumoral prostate tissue compared with the non-tumor tissue.
    Experimental approach: 43 pairs of prostate tumoral and non-tumor tissue were prepared. The expression levels of JPX and LINC00641 were investigated by RT-qPCR.
    Findings/Results: Significant upregulation of LINC00641 (2.47 +/- 0.5 vs 1.41 +/- 0.2) and downregulation of JPX (1.42 +/- 0.6 vs 2.83 +/- 1.0) were observed in PC tissues compared with the normal tissues (their adjacent non-tumoral tissues).
    Conclusion and implications: Dysregulation of JPX and LINC00641 in PC patients could be used in the future as a prognostic biomarker in PC.
    Accession Number: WOS:000687206500006
    PubMed ID: 34522197
    ISSN: 1735-5362
    eISSN: 1735-9414
    Record 4 of 17
    Title: A Novel Mutation in the VPS13B Gene in a Cohen Syndrome Patient with Positive Antiphospholipid Antibodies
    Author(s): Dehghan, R (Dehghan, Roghayeh); Behnam, M (Behnam, Mahdiyeh); Moafi, A (Moafi, Alireza); Salehi, M (Salehi, Mansoor)
    Source: CASE REPORTS IN IMMUNOLOGY  Volume: 2021  Article Number: 3143609  DOI: 10.1155/2021/3143609  Published: AUG 26 2021  
    Abstract: Cohen syndrome is an autosomal recessive disorder with the primary symptoms of mental deficiency, progressive retinopathy, hypotonia, microcephaly, obesity of midchildhood onset, intermittent neutropenia, and dysmorphic facial features. The syndrome has high phenotypic heterogeneity and is caused by loss-of-function mutations in the VPS13B gene. Here, we introduce a novel homozygous nonsense mutation (c.8698G > T, p.E2900X) in the VPS13B gene in an 11-year-old Iranian boy with major symptoms of Cohen syndrome. He also had mild anemia accompanied by positive antiphospholipid antibodies, the latter has never been previously reported in Cohen syndrome.
    Accession Number: WOS:000692886600001
    PubMed ID: 34484844
    Author Identifiers:
    Salehi, Mansoor    0000-0002-6565-0907 
    ISSN: 2090-6609
    eISSN: 2090-6617
    Record 5 of 17
    Title: Strong association of common variants in the miRNA-binding site of NOD2 gene with clinicopathological characteristics and disease activity of systemic lupus erythematosus
    Author(s): Esmaeilzadeh, E (Esmaeilzadeh, Emran); Saghi, M (Saghi, Mostafa); Hassani, M (Hassani, Mehdi); Davar, S (Davar, Saeideh); Alani, B (Alani, Behrang); Pakzad, B (Pakzad, Bahram); Ghobakhloo, S (Ghobakhloo, Sepideh); Khosravi, S (Khosravi, Sharifeh); Sabet, MN (Sabet, Mehrdad Nasrollahzadeh)
    Source: CLINICAL RHEUMATOLOGY  Volume: 40  Issue: 11  Pages: 4559-4567  DOI: 10.1007/s10067-021-05812-6  Early Access Date: JUN 2021   Published: NOV 2021  
    Abstract: Introduction/objectives Systemic lupus erythematosus (SLE) is a multifactorial systemic autoimmune disease, in which genetic susceptibility plays a pivotal role. The nucleotide oligomerization domain 2 (NOD2) gene is one of the main regulators of chronic inflammatory conditions and could be involved in SLE pathogenesis. Single nucleotide polymorphisms (SNPs) in miRNA binding sites which are located in 3'UTR of the NOD2 gene could be associated with SLE risk by dysregulation of NOD2 expression. In the present study, we assessed the possible association between SNPs rs3135500 and rs3135499 in the NOD2 gene with SLE risk in the Iranian population.
    Methods A case-control study using 110 SLE patients and 120 control subjects was undertaken to estimate rs3135500 (G > A) and rs3135499 (A > C) genotypes via real-time PCR high-resolution melting method (HRM).
    Results No significant association was observed between allele and genotype frequencies of rs3135500 and rs3135499 polymorphisms and SLE risk in this population (P > 0.05). However, there was an obvious association between rs3135500 (A allele) with laboratory factors that are associated with disease activity (P < 0.05) and some clinical manifestations that are associated with disease severity such as neurological symptoms, skin manifestations, renal involvements, and higher serum concentration of creatinine (P < 0.05). Besides, rs3135499 (C allele) was correlated with renal involvement and also the concentration of creatinine (P < 0.05). Moreover, in the patients group, the risk alleles in these polymorphisms were associated with lower age of onset (P < 0.05).
    Conclusions Our results suggest a substantial association between NOD2 polymorphisms with clinicopathological characteristics and SLE disease activity.
    Accession Number: WOS:000667904100001
    PubMed ID: 34173079
    Author Identifiers:
    alani, behrang  G-4477-2016  0000-0002-1006-1144 
    ISSN: 0770-3198
    eISSN: 1434-9949
    Record 6 of 17
    Title: WRN Germline Mutation Is the Likely Inherited Etiology of Various Cancer Types in One Iranian Family
    Author(s): Norouzi, M (Norouzi, Mahnaz); Shafiei, M (Shafiei, Mohammad); Abdollahi, Z (Abdollahi, Zeinab); Miar, P (Miar, Paniz); Galehdari, H (Galehdari, Hamid); Emami, MH (Emami, Mohammad Hasan); Zeinalian, M (Zeinalian, Mehrdad); Tabatabaiefar, MA (Tabatabaiefar, Mohammad Amin)
    Source: FRONTIERS IN ONCOLOGY  Volume: 11  Article Number: 648649  DOI: 10.3389/fonc.2021.648649  Published: JUN 7 2021  
    Abstract: Background
    Familial cancers comprise a considerable distribution of colorectal cancers (CRCs), of which only about 5% occurs through well-established hereditary syndromes. It has been demonstrated that deleterious variants at the newly identified cancer-predisposing genes could describe the etiology of undefined familial cancers.
    Methods
    The present study aimed to identify the genetic etiology in a 32-year-old man with early onset familial CRC employing several molecular diagnostic techniques. DNA was extracted from tumoral and normal formalin-fixed-paraffin-embedded (FFPE) blocks, and microsatellite instability (MSI) was evaluated. Immunohistochemistry staining of MMR proteins was performed on tumoral FFPE blocks. Next-generation sequencing (NGS), multiplex ligation-dependent amplification (MLPA) assay, and Sanger sequencing were applied on the genomic DNA extracted from peripheral blood. Data analysis was performed using bioinformatics tools. Genetic variants interpretation was based on ACMG.
    Results
    MSI analysis indicated MSI-H phenotype, and IHC staining proved no expressions of MSH2 and MSH6 proteins. MLPA and NGS data showed no pathogenic variants in MMR genes. Further analysis of NGS data revealed a candidate WRN frameshift variant (p.R389Efs*3), which was validated with Sanger sequencing. The variant was interpreted as pathogenic since it met the criteria based on the ACMG guideline including very strong (PVS1), strong (PS3), and moderate (PM2).
    Conclusion
    WRN is a DNA helicase participating in DNA repair pathways to sustain genomic stability. WRN deficient function may contribute to CRC development that is valuable for further investigation as a candidate gene in hereditary cancer syndrome diagnosis.
    Accession Number: WOS:000663748700001
    PubMed ID: 34164337
    ISSN: 2234-943X
    Record 7 of 17
    Title: Association of serum and follicular fluid leptin and in vitro Fertilization/ICSI outcome: A systematic review and meta-analysis
    Author(s): Jafarpour, S (Jafarpour, Sima); Khosravi, S (Khosravi, Sharifeh); Janghorbani, M (Janghorbani, Mohsen); Mansourian, M (Mansourian, Marjan); Karimi, R (Karimi, Raheleh); Ghiasi, MR (Ghiasi, Moosa Rahimi); Miraghajani, M (Miraghajani, Maryam); Symonds, ME (Symonds, Michael E.); Farajzadeghan, Z (Farajzadeghan, Ziba); Salehi, R (Salehi, Rasoul)
    Source: JOURNAL OF GYNECOLOGY OBSTETRICS AND HUMAN REPRODUCTION  Volume: 50  Issue: 6  Article Number: 101924  DOI: 10.1016/j.jogoh.2020.101924  Published: JUN 2021  
    Abstract: There are conflicting reports regarding circulating leptin and its relationship between pregnancy outcomes in infertile women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). We performed a systematic review and meta-analysis to assess the association between serum or follicular fluid (FF) leptin concentrations reported for infertile women and their IVF outcome. A systematic search was undertaken in available databases (PubMed, Scopus, Web of Science, The Cochrane Library and Embase) to find studies published up to Aug 2020 and the standardized mean difference with 95 % confidence interval was taken from 14 eligible studies. Both graphical (funnel plots) and test methods (Egger's regression test and the Begg) assessedthepresence of publicationbias. Subgroup analysis was used to investigate the source of heterogeneity. Pooled effect sizes based on the eligible papers indicated that of there is no statistically significant correlation between leptin levels in follicular fluid and serum on the day of ovum pick-up (OPU) and day of HCG (human chorionic gonadotrophin) administration in pregnant and non-pregnant women who underwent IVF/ICSI cycles. However, combination of leptin in serum and/or FF with other parameters may be a useful marker to predict IVF outcome. (C) 2020 Elsevier Masson SAS. All rights reserved.
    Accession Number: WOS:000653997500021
    PubMed ID: 33007525
    ISSN: 2468-7847
    eISSN: 1773-0430
    Record 8 of 17
    Title: Three functional variants in the NLRP3 gene are associated with susceptibility and clinical characteristics of systemic lupus erythematosus
    Author(s): Ehtesham, N (Ehtesham, Naeim); Rafie, MZ (Rafie, Maryam Zare); Esmaeilzadeh, E (Esmaeilzadeh, Emran); Dehani, M (Dehani, Mohammad); Davar, S (Davar, Saeideh); Mosallaei, M (Mosallaei, Meysam); Pakzad, B (Pakzad, Bahram); Ghorashi, T (Ghorashi, Tahereh); Darvish, H (Darvish, Hossein); Soosanabadi, M (Soosanabadi, Mohsen)
    Source: LUPUS  Volume: 30  Issue: 8  Pages: 1273-1282  Article Number: 09612033211014273  DOI: 10.1177/09612033211014273  Early Access Date: MAY 2021   Published: JUL 2021  
    Abstract: Objective
    Nod-like receptor pyrin domain containing 3 (NLRP3) gene encodes an intracellular receptor whose dysregulation in systemic lupus erythematosus (SLE) has been reported in multiple studies. Activation of NLRP3 inflammasome leads to the induction of inflammatory response via cleaving and producing of specific cytokines. In the present study, we assessed the possible association between three functional polymorphisms in this gene and SLE risk in the Iranian population. These variants include two gain of function (rs4612666 and rs10754558) and one loss of function (rs6672995) which are correlated with modulation of expression of NLRP3.
    Methods
    A case-control study involving 110 SLE patients and 116 control subjects was undertaken to estimate the frequency of rs4612666, rs10754558, and rs6672995 genotypes using real-time PCR high resolution melting method (HRM).
    Results
    Our findings revealed significant associations between GG genotype and G allele of rs10754558 with increased risk of SLE (OR (for GG genotype)= 2.82; 95%CI [1.45-5.46]/OR (for G allele)= 1.97; 95%CI [1.36-2.87]). Although, no significant associations were recognized between allele and genotype frequencies of rs4612666 and rs6672995 polymorphisms with SLE risk (P > 0.05). Also, our analysis revealed that the C allele in rs4612666 and G allele in rs10754558 was correlated with the severity of disease activity (P < 0.001). Moreover, these common variants were associated with lower age of onset and some clinical symptoms in the patient group, such as skin manifestation, neurological symptom and, renal involvement (P < 0.05).
    Conclusion
    This study demonstrates a substantial association between NLRP3 polymorphisms with increased risk, clinical symptoms, and the severity of disease activity of SLE.
    Accession Number: WOS:000651160300001
    PubMed ID: 33951966
    Author Identifiers:
    Ehtesham, Naeim    0000-0002-1769-6329 
    dehani, mohammad    0000-0001-8774-1255 
    ISSN: 0961-2033
    eISSN: 1477-0962
    Record 9 of 17
    Title: The dysbiosis signature of Fusobacterium nucleatum in colorectal cancer-cause or consequences? A systematic review
    Author(s): Ranjbar, M (Ranjbar, Maryam); Salehi, R (Salehi, Rasoul); Javanmard, SH (Haghjooy Javanmard, Shaghayegh); Rafiee, L (Rafiee, Laleh); Faraji, H (Faraji, Habibollah); Jafarpor, S (Jafarpor, Sima); Ferns, GA (Ferns, Gordon A.); Ghayour-Mobarhan, M (Ghayour-Mobarhan, Majid); Manian, M (Manian, Mostafa); Nedaeinia, R (Nedaeinia, Reza)
    Source: CANCER CELL INTERNATIONAL  Volume: 21  Issue: 1  Article Number: 194  DOI: 10.1186/s12935-021-01886-z  Published: APR 6 2021  
    Abstract: Colorectal cancer (CRC) is the third most common cause of cancer globally and the fourth attributable cause of mortality and morbidity due to cancer. An emerging factor contributing to CRC is the gut microbiota and the cellular changes associated with it. Further insights on this may help in the prevention, diagnosis and new therapeutic approaches to colorectal cancer. In most cases of CRC, genetic factors appear to contribute less to its aetiology than environmental and epigenetic factors; therefore, it may be important to investigate these environmental factors, their effects, and the mechanisms that may contribute to this cancer. The gut microbiota has recently been highlighted as a potential risk factor that may affect the structural components of the tumor microenvironment, as well as free radical and enzymatic metabolites directly, or indirectly. Many studies have reported changes in the gut microbiota of patients with colorectal cancer. What is controversial is whether the cancer is the cause or consequence of the change in the microbiota. There is strong evidence supporting both possibilities. The presence of Fusobacterium nucleatum in human colorectal specimens has been demonstrated by RNA-sequencing. F. nucleatum has been shown to express high levels of virulence factors such as FadA, Fap2 and MORN2 proteins. Our review of the published data suggest that F. nucleatum may be a prognostic biomarker of CRC risk, and hence raises the potential of antibiotic treatment of F. nucleatum for the prevention of CRC.
    Accession Number: WOS:000637500700001
    PubMed ID: 33823861
    Author Identifiers:
    Javanmard, Shaghayegh Haghjooy  W-5060-2017  0000-0002-3853-5006 
    Rafiee, Laleh  AAT-4442-2021   
    Nedaeinia, Reza    0000-0001-9922-7181 
    eISSN: 1475-2867
    Record 10 of 17
    Title: Association of rs3135500 and rs3135499 Polymorphisms in the MicroRNA-binding Site of Nucleotide-binding Oligomerization Domain 2 (NOD2) Gene with Susceptibility to Rheumatoid Arthritis
    Author(s): Ehtesham, N (Ehtesham, Naeim); Alani, B (Alani, Behrang); Mortazavi, D (Mortazavi, Deniz); Azhdari, S (Azhdari, Sara); Kenarangi, T (Kenarangi, Taiebe); Esmaeilzadeh, E (Esmaeilzadeh, Emran); Pakzad, B (Pakzad, Bahram)
    Source: IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY  Volume: 20  Issue: 2  Pages: 178-187  DOI: 10.18502/ijaai.v20i2.6051  Published: APR 2021  
    Abstract: The nucleotide-binding oligomerization domain 2 (NOD2) is the key regulator of inflammatory responses and has been involved in the pathogenesis of rheumatoid arthritis (RA). Laboratory and in silico evaluations have demonstrated that some polymorphisms in 3'UTR of NOD2 gene could influence the secondary structure of this region and similarly thermodynamic features of hybridization site and finally deregulate the expression of NOD2. In the current study, for the first time, we evaluated the possible association between single nucleotide polymorphisms (SNPs) rs3135500 and rs3135499 in the NOD2 gene with RA risk in the Iranian population.
    One hundred and fifteen patients with RA and 120 healthy subjects were recruited in this case-control study. Genotyping of rs3135500 and rs3135499 polymorphisms were accomplished using the real-time polymerase chain reaction high resolution melting (HRM) method.
    We found a substantial association of AA and AG genotypes in rs3135500 with the risk of RA (AA vs GG; OR=5.547; 95%CI [2.564-11.999]; p<0.001 and AG vs GG; OR=2.179; 95%CI [1.145-4.147]; p=0.017). Moreover, in the patient group, there was a significant relationship between the increased concentration of erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) with rs3135500 (A allele) (p<0.05). However, there were no important associations between rs3135499 with the risk of RA (p>0.05). However, we found a noteworthy association of the C allele in rs3135499 with an increased level of CRP in patients (p>0.05).
    Our findings propose a considerable association between NOD2 polymorphisms with increased risk of RA and disease activity.
    Accession Number: WOS:000645052000006
    PubMed ID: 33904676
    Author Identifiers:
    Ehtesham, Naeim    0000-0002-1769-6329 
    alani, behrang  G-4477-2016  0000-0002-1006-1144 
    ISSN: 1735-1502
    eISSN: 1735-5249
    Record 11 of 17
    Title: Nervous System Involvement in COVID-19: a Review of the Current Knowledge
    Author(s): Norouzi, M (Norouzi, Mahnaz); Miar, P (Miar, Paniz); Norouzi, S (Norouzi, Shaghayegh); Nikpour, P (Nikpour, Parvaneh)
    Source: MOLECULAR NEUROBIOLOGY  Volume: 58  Issue: 7  Pages: 3561-3574  DOI: 10.1007/s12035-021-02347-4  Early Access Date: MAR 2021   Published: JUL 2021  
    Abstract: The current pandemic of the new human coronavirus (CoV), i.e., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has created an urgent global condition. The disease, termed coronavirus disease 2019 (COVID-19), is primarily known as a respiratory tract infection. Although SARS-CoV-2 directly invades the lungs, COVID-19 is a complex multi-system disease with varying degrees of severity and affects several human systems including the cardiovascular, respiratory, gastrointestinal, neurological, hematopoietic, and immune systems. From the existing data, most COVID-19 cases develop a mild disease typically presented with fever and respiratory illness. However, in some patients, clinical evidence suggests that COVID-19 might progress to acute respiratory distress syndrome (ARDS), multi-organ dysfunction, and septic shock resulting in a critical condition. Likewise, specific organ dysfunction seems to be related to the disease complication, worsens the condition, and increases the lethality of COVID-19. The neurological manifestations in association with disease severity and mortality have been reported in COVID-19 patients. Despite the continuously increasing reports of the neurological symptoms of SARS-CoV-2, our knowledge about the possible routes of nervous system involvement associated with COVID-19 is limited. Herein, we will primarily describe the critical aspects and clinical features of SARS-CoV-2 related to nervous system impairment and then discuss possible routes of SARS-CoV-2 nervous system involvement based on the current evidence.
    Accession Number: WOS:000632758900001
    PubMed ID: 33765290
    Author Identifiers:
    Nikpour, Parvaneh    0000-0002-1784-6040 
    ISSN: 0893-7648
    eISSN: 1559-1182
    Record 12 of 17
    Title: Assessment of heavy metal contamination in herbal medicinal products consumed in the Iranian market
    Author(s): Keshvari, M (Keshvari, Mahtab); Nedaeinia, R (Nedaeinia, Reza); Nedaeinia, M (Nedaeinia, Mozhdeh); Ferns, GA (Ferns, Gordon A.); Nia, SN (Nia, Sasan Nedaee); Asgary, S (Asgary, Sedigheh)
    Source: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH  Volume: 28  Issue: 25  Pages: 33208-33218  DOI: 10.1007/s11356-021-13020-7  Early Access Date: FEB 2021   Published: JUL 2021  
    Abstract: Herbal medicines have been reported to contain many contaminants that are potential harmful to health. These include heavy metals, such as lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg). Therefore, this study aimed to measure the levels of Pb, Cd, As, and Hg in several orally administered herbal products available in the Iranian market. Fifteen products labeled (A-O) of different brands from three different production batches (n = 45) were purchased from traditional herbal medicine factories in Iran. Each sample was digested with nitric acid by the wet digestion method, and the resultant solutions were used to determine the concentrations of Pb, Cd, As, and Hg. These measurements were performed using flame atomic absorption spectrometry, graphite furnace atomic absorption, or cold vapor atomic absorption. The lead, cadmium, arsenic, and mercury contents in the investigated samples did not show significant levels that may be associated with toxicity. All four metals were present at concentrations below the limits recommended by the WHO (World Health Organization), American Herbal Products Association (AHPA), and Canadian standard, but in several herbal products, the concentrations of these trace elements exceeded the Agency for Toxic Substances and Disease Registry (ATSDR). The concentrations of Pb, Cd, As, and Hg in commercially available herbal remedies were well below the acceptable intake recommended by global recommendations. Our findings revealed that at present, the amount of heavy metals in medicinal herbs processed at the level of supply by pharmacies licensed is favorable.
    Accession Number: WOS:000622284400001
    PubMed ID: 33638076
    Author Identifiers:
    Nedaeinia, Reza    0000-0001-9922-7181 
    ISSN: 0944-1344
    eISSN: 1614-7499
    Record 13 of 17
    Title: The impact of neutrophil extracellular trap from patients with systemic lupus erythematosus on the viability, CD11b expression and oxidative burst of healthy neutrophils
    Author(s): Fatemi, A (Fatemi, Alimohammad); Alipour, R (Alipour, Razieh); Khanahmad, H (Khanahmad, Hossein); Alsahebfosul, F (Alsahebfosul, Fereshteh); Andalib, A (Andalib, Alireza); Pourazar, A (Pourazar, Abbasali)
    Source: BMC IMMUNOLOGY  Volume: 22  Issue: 1  Article Number: 12  DOI: 10.1186/s12865-021-00402-2  Published: FEB 5 2021  
    Abstract: Background NET (neutrophil extracellular trap) has been shown to directly influence inflammation; in SLE (systemic lupus erythematosus), it is reportedly a plausible cause for the broken self-tolerance that contributes to this pathology. Meanwhile, the role of NET is not easily explicable, and there is a serious discrepancy in the role of NET in SLE pathology and generally inflammation; in particular, the interactions of neutrophils with NET have been rarely inspected. This study evaluates the effect of NET on neutrophils in the context of SLE. The neutrophils were incubated by the collected NET (from SLE patients and healthy controls) and their expression of an activation marker, viability and oxidative burst ability were measured. Results The level of cell mortality, CD11b expression and the oxidative burst capacity were elevated in NET-treated neutrophils. Also, the elevation caused by the SLE NET was higher than that produced by the healthy NET. Conclusion The decreased neutrophil viability was not due to the increase in apoptosis; rather, it was because of the augmentation of other inflammatory cell-death modes. The upregulation of CD11b implies that NET causes neutrophils to more actively contribute to inflammation. The increased oxidative burst capacity of neutrophils can play a double role in inflammation. Overall, the effects induced by NET on neutrophils help prolong inflammation; accordingly, the NET collected from SLE patients is stronger than the NET from healthy individuals.
    Accession Number: WOS:000615242300001
    PubMed ID: 33546594
    eISSN: 1471-2172
    Record 14 of 17
    Title: Molecular diagnosis of SLC26A4-related hereditary hearing loss in a group of patients from two provinces of Iran
    Author(s): Koohiyan, M (Koohiyan, Mahbobeh); Hashemzadeh-Chaleshtori, M (Hashemzadeh-Chaleshtori, Morteza); Tabatabaiefar, MA (Tabatabaiefar, Mohammad Amin)
    Source: INTRACTABLE & RARE DISEASES RESEARCH  Volume: 10  Issue: 1  Pages: 23-30  DOI: 10.5582/irdr.2020.03090  Published: FEB 2021  
    Abstract: The SLC26A4 gene has been described as the second gene involved in most cases of autosomal recessive non-syndromic hearing loss (ARNSHL), after GJB2. Over 500 different SLC26A4 mutations have been reported, with each ethnic population having its own distinctive mutations. Here, we aimed to determine the frequency and mutation profile of the SLC26A4 gene from two different provinces (center and west) of Iran. This study included 50 nuclear families with two or more siblings segregating presumed ARNSHL. All affected tested negative for mutations in GJB2 at the DFNB1 locus and were therefore screened for autozygosity by descent using short tandem repeat polymorphisms (STRPs) of DFNB4. Sanger sequencing was performed to screen the 20 exons of the SLC26A4 gene for the families linked to this locus. In silico analyses were also performed using available software tools. Four out of 25 (16%) and 3 of 25 (12%) studied families of Isfahan and Hamedan provinces, respectively. were linked to DFNB4. Sanger sequencing led to the identification of six different mutations, one of which (c.919-2A>G) was recurrent and accounted for 31% of all mutant alleles. One out of 7 (14.3%) families with mutations were confirmed to be Pendred syndrome (PS). The SLC26A4 mutations have a high carrying rate in ARNSHL Iranian patients. The identification of a disease causing mutation can be used to establish a genotypic diagnosis and provide important information to the patients and their families.
    Accession Number: WOS:000615783500004
    PubMed ID: 33614372
    ISSN: 2186-3644
    eISSN: 2186-361X
    Record 15 of 17
    Title: Next-generation sequencing reveals a novel pathogenic variant in the ATM gene
    Author(s): Pourahmadiyan, A (Pourahmadiyan, Azam); Alipour, P (Alipour, Paria); Golchin, N (Golchin, Neda); Tabatabaiefar, MA (Tabatabaiefar, Mohammad Amin)
    Source: INTERNATIONAL JOURNAL OF NEUROSCIENCE  DOI: 10.1080/00207454.2020.1826944  Early Access Date: JAN 2021   
    Abstract: Introduction
    Ataxia telangiectasia (A-T) is a rare autosomal recessive, multisystemic disease. Patients with the A-T syndrome present a broad spectrum of disease phenotypes. The ATM (ataxia telangiectasia mutated) gene, the only causative gene for A-T.
    Method
    A patient of Persian origin presenting with typical A-T was referred to our genetics centre for specialized genetic counselling and testing. Targeted next-generation sequencing (NGS) was applied. Sanger sequencing was used to confirm the candidate variant. Modelling was performed using the SWISS-MODEL server.
    Results
    A homozygous stop-gain variant c.829G > T (p.E277*) was found in the ATM gene. This variant was confirmed by Sanger sequencing and modelling of native structure, and truncated structure was performed.
    Conclusion
    To date, very few pathogenic variants of the ATM gene have been reported from the Iranian population. The finding has implications in molecular diagnostic for A-T in Iran.
    Accession Number: WOS:000611648300001
    PubMed ID: 32962506
    ISSN: 0020-7454
    eISSN: 1563-5279
    Record 16 of 17
    Title: The role of hypoxia in the tumor microenvironment and development of cancer stem cell: a novel approach to developing treatment
    Author(s): Nejad, AE (Nejad, Asieh Emami); Najafgholian, S (Najafgholian, Simin); Rostami, A (Rostami, Alireza); Sistani, A (Sistani, Alireza); Shojaeifar, S (Shojaeifar, Samaneh); Esparvarinha, M (Esparvarinha, Mojgan); Nedaeinia, R (Nedaeinia, Reza); Javanmard, SH (Javanmard, Shaghayegh Haghjooy); Taherian, M (Taherian, Marjan); Ahmadlou, M (Ahmadlou, Mojtaba); Salehi, R (Salehi, Rasoul); Sadeghi, B (Sadeghi, Bahman); Manian, M (Manian, Mostafa)
    Source: CANCER CELL INTERNATIONAL  Volume: 21  Issue: 1  Article Number: 62  DOI: 10.1186/s12935-020-01719-5  Published: JAN 20 2021  
    Abstract: Hypoxia is a common feature of solid tumors, and develops because of the rapid growth of the tumor that outstrips the oxygen supply, and impaired blood flow due to the formation of abnormal blood vessels supplying the tumor. It has been reported that tumor hypoxia can: activate angiogenesis, thereby enhancing invasiveness and risk of metastasis; increase survival of tumor, as well as suppress anti-tumor immunity and hamper the therapeutic response. Hypoxia mediates these effects by several potential mechanisms: altering gene expression, the activation of oncogenes, inactivation of suppressor genes, reducing genomic stability and clonal selection. We have reviewed the effects of hypoxia on tumor biology and the possible strategiesto manage the hypoxic tumor microenvironment (TME), highlighting the potential use of cancer stem cells in tumor treatment.
    Accession Number: WOS:000612652900004
    PubMed ID: 33472628
    Author Identifiers:
    sedeh, bahman sadeghi  AAT-9409-2021  0000-0002-1667-6879 
    Javanmard, Shaghayegh Haghjooy  W-5060-2017  0000-0002-3853-5006 
    Nedaeinia, Reza    0000-0001-9922-7181 
    eISSN: 1475-2867
    Record 17 of 17
    Title: Possible Preventive Effect of Donepezil and Hyoscyamoside by Reduction of Plaque Formation and Neuroinflammation in Alzheimer's Disease
    Author(s): Soureshjani, FH (Soureshjani, Fatemeh Heidari); Kheirollahi, M (Kheirollahi, Majid); Yaghmaei, P (Yaghmaei, Parichehreh); Jadnematalahi, FS (Jadnematalahi, Fattah Sotoodehne)
    Source: INTERNATIONAL JOURNAL OF PREVENTIVE MEDICINE  Volume: 12  Issue: 1  DOI: 10.4103/ijpvm.IJPVM_143_19  Published: JAN-DEC 2021  
    Abstract: Background: Alzheimer disease (AD) is the most common age-dependent dementia. The complex natural accumulation of amyloid beta (A beta) precursor protein in hippocampus neurons is regarded as the earliest pathological feature of AD, although there are cholinergic assumptions and effective inflammation in AD. In this animal experimental study, we evaluated the preventive effect of hyoscyamoside (Hyo) and donepezil (Dz) on plaque formation and improvement of neurogenic inflammation in AD rats. Methods: Dz was prepared and Hyo (steroidal saponin) was isolated from Hyoscymus niger. Then, Wistar rats divided into five groups including negative and positive controls, AD, Dz, and Hyo treatment groups based on the drug exposure and their behavioral alternation was examined using Morris water maze (MWM) test. Bielschowsky staining was used to detect the nerve fibers. Serum levels of interleukin (IL)-4 and IL-6 were evaluated by ELISA. The RNA expression of cyclin-dependent kinase CDK11-P58 in peripheral blood lymphocytes was performed using quantitative PCR. Results: The MWM test showed significant changes in time the models spent to find the hidden platform. The Hyo treatment group showed a notable speed change (P < 0.01). The histopathological analysis of the hippocampal tissue revealed the inhibition of A beta formation in the treatment groups. The treatment groups had a significant decline in the serum level of IL-6, and the IL-4 serum level was increased in the Hyo and Dz treated groups. The expression levels of CDK11-P58 was significantly decreased in the treatment groups. Conclusions: In sum, the therapeutic effects of Hyo is comparable with that of Dz in AD rats by suppressing neuroinflammation. Thus, these compounds could be considered as a preventive agent in the AD therapy.
    Accession Number: WOS:000673028500009
    PubMed ID: 34447508
    ISSN: 2008-7802
    eISSN: 2008-8213

 

  • Book
  • Epigenetics and Childhood Obesity , Chapter: CHILDHOOD OBESITY CAUSES, PREVENTION AND MANAGEMENT Publication .  Nova Science
Prepared at Isfahan University of Medical Sciences