DNA methylation and gene expression in children

Cells from the same individual share the same genome but do not express the same genes. Each cell type expresses a different set of genes, which allows it to exert a unique function. This is regulated by the epigenome -- chemical modifications of genome (including DNA methylation), which can turn genes on or off. Besides its central role in regulating development, the epigenome also allows cells to respond to environmental factors. Recently, many studies (called epigenome-wide association studies) have tried to link exposure to environmental factors with altered DNA methylation patterns and disease. However, most analyses do not allow to determine if and how these methylated sites affect the expression of nearby genes.

In this study, the research team aimed to identify associations between DNA methylation levels at particular positions of the genome with the expression of nearby genes, called expression quantitative trait methylation or eQTMs, using blood samples from 832 children, aged 6-11 years, of the Human Early Life Exposome (HELIX) project. Firstly, methylation and gene expression levels were assessed for each sample. Secondly, each gene was paired with the closest methylation site or sites. The analysis identified almost 40,000 significant associations between methylation levels and gene expression. In most cases, the methylation site was quite close to the regulated gene, and in 59% of cases, there was an inverse relationship between methylation and gene expression levels (meaning higher levels of methylation -- lower gene expression). Only 13.8% of the associations identified were also found in adults. "This was expected, as it is known that DNA methylation and gene expression change with age, however differences in study designs can also explain this," explains Carlos Ruiz-Arenas, first author of the study.

Reference:

Carlos Ruiz-Arenas, Carles Hernandez-Ferrer, Marta Vives-Usano, Sergi Marí, Ines Quintela, Dan Mason, Solène Cadiou, Maribel Casas, Sandra Andrusaityte, Kristine Bjerve Gutzkow, Marina Vafeiadi, John Wright, Johanna Lepeule, Regina Grazuleviciene, Leda Chatzi, Ángel Carracedo, Xavier Estivill, Eulàlia Marti, Geòrgia Escaramís, Martine Vrijheid, Juan R González, Mariona Bustamante. Identification of autosomal cis expression quantitative trait methylation (cis eQTMs) in children’s blood. eLife, 2022; 11 DOI: 10.7554/eLife.65310